RISK-HUNT3R - RISK assessment of chemicals integrating HUman centric Next generation Testing strategies promoting the 3Rs
Funding by: EU
Funding period: 2021 - 2026
Coordinator: Leiden University, The Netherlands
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Project website:
External Link:https://www.risk-hunt3r.eu/
EU information:
Aim
To develop human-relevant test methods as well as test strategies for the classification of chemicals, independent of the availability of additional animal test data. The focus is on the development of concepts and strategies for the investigation of i) specific target organ toxicity (STOT) after single and repeated (chronic) exposure; ii) developmental neurotoxicity (DNT) and iii) non-genotoxic carcinogenesis (NGTxC). For these complex, systemic, toxic effects in particular, there are neither sufficient methods nor concepts available to effectively reduce animal testing.
3R Relevance
Promote the transition to non-animal risk assessment of chemicals using human-centered testing and assessment strategies.
Literatur
Wedler, M., Körber, N., Liu, S. (2026). Analyzing Cell Painting PLUS Data Using Cell Profiler and KNIME Analytics Platform. In: Dunst, S., Fetz, V. (eds) Phenotypic Screening. Methods in Molecular Biology, vol 2989. Humana, New York, NY. External Link:https://doi.org/10.1007/978-1-0716-4985-5_3
von Coburg, E., Wedler, M., Muino, J.M., Wolff, C., Körber, N., Dunst, S. & Liu, S. (2025) Cell Painting PLUS: expanding the multiplexing capacity of Cell Painting-based phenotypic profiling using iterative staining-elution cycles. Nat Commun 16, 3857 (2025). External Link:https://doi.org/10.1038/s41467-025-58765-8
Wedler, M., von Coburg, E., Muino, J. M., Valentin, L., Körber, N., Dunst, S., & Liu, S. (2026) Cell Painting PLUS: An Iterative Staining-Elution Protocol for High-Content Phenotypic Screenings. Curr Protoc, 6(4), e70368. External Link:https://doi.org/10.5281/zenodo.18184315
von Coburg, E., Wedler, M., Muino, J. M., Wolff, C., Körber, N., Dunst, S. & Liu, S. (2025) Cell Painting PLUS: expanding the multiplexing capacity of Cell Painting-based phenotypic profiling using iterative staining-elution cycles. Nat Commun 16, 3857 (2025) External Link:https://zenodo.org/records/14982928